Replication data for: Comparative evaluation of DNS, PAHBAH, and BCA colourimetric assays for quantifying reducing sugars

This dataset contains all obtained data of the publication "Comparative evaluation of DNS, PAHBAH, and BCA colourimetric assays for quantifying reducing sugars" by Lisa Coddens, Charlotte F. De Schepper, Kristof Brijs and Christophe M. Courtin (https://doi.org/10.1016/j.carres.2025.109770). In this publication, three colourimetric assays were compared for quantifying reducing sugars to evaluate glycoside hydrolase activity. The DNS and PAHBAH assays overestimated reducing sugar concentrations, with the extent of overestimation increasing with the degree of polymerisation, attributed to alkaline degradation. In contrast, the BCA assay showed minimal alkaline degradation, and its measurements were independent of degree of polymerisation, making it a promising assay for reliable, high-throughput screening.