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Chlamydomonas reinhardtii quantification of Mannosidase 1A and Xylosyltransferase1A

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NIAID Data Ecosystem2026-03-10 收录
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We have identified insertional mutants of mannosidase 1A and xylosyltransferase 1A in Chlamydomonas reinhardtii. To verify a knockout/knockdown of the enzymes in the insertional mutant strains, PRM measurements were performed. Mass spectrometric analyses of intact N-glycopeptides revealed that disruption of the genes altered the N-glycan composition of the alga (in single as well as double mutant).

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2018-02-27
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