Primer, adaptor and sample-specific barcode sequences.
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The reverse primers have two degenerate nucleotide positions where R denominates A/G. The sequencing reaction is primed by an oligonucleotide complementary to the adaptor sequence of the reverse primer, such that the barcode sequence will be read first, followed by the primer sequence, followed by the variable 16S rDNA sequence.
创建时间：
2008-07-30
