Pristis pectinata RNAseq

RNA was extracted from the rostral skin, brain, liver, ovary, and kidney tissues collected from a female smalltooth sawfish via TRIzol. Samples (~100 mg) of each tissue type were homogenized in 1ml TRIzol and incubated at 20C for 10 mins, followed by clarification by centrifugation. Supernatant was combined with chloroform and phase separated. The aqueous phase was precipitated in isopropanol, the pellet washed in 70% ethanol, and suspended in nuclease free water. The concentrations and purities of RNA extracts were assessed by nanodropand through a bioanalyzer 2100. Total RNA extracts from each tissue type (rostral, skin, brain, liver, ovary, kidney) were sequenced by Novogene. Prior to sequencing, libraries were subject to rigorous QC to validate library creation. RNA sequencing was carried out using libraries produced with the Illumina TruSeq RNA Prep Kit v2.