High-throughput nanopore targeted sequencing for efficient drug resistance assay of Mycobacterium tuberculosis

Drug-resistant tuberculosis, especially multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis, is one of the urgent clinical problems and public health challenges. To simultaneously assay the drug resistance of Mycobacterium tuberculosis against first-line and second-line anti-TB drugs, we developed a method based on the nanopore sequencing technology combined with multiplex PCR, namely nanopore targeted sequencing.