Additional file 4 of Sampling the fish gill microbiome: a comparison of tissue biopsies and swabs

Additional file 4. UNIFRAC nmMDS. Filtered sequences were aligned using MAFFT [110], and phylogenetic tree built using FASTTREE [111] using default parameters within the QIIME2 pipeline. The resultant UNIFRAC distance matrix was used in generation of non-metric multidimensional scaling analysis as shown here. Results of PERMANOVA using unrestricted permutations for comparison of samples by sample type (swab;biopsy;environmental) shown in table indicate significant variation by sample type.