Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice

Short-term repeated intranasal exposure crystalline silica (cSiO2), a known human autoimmune trigger, induces uncontrolled inflammation, upregulated IFN-stimulated gene expression, diverse autoantibody production, and glomerulonephritis in lupus-prone female NZBWF1 mice. Dietary supplementation with the omega-3 fatty acid docosahexaenoic acid (DHA) prevents subchronic cSiO2 triggering of these lupus hallmarks. To understand how this intervention impacts acute effects of cSiO2, we fed NZBWF1 mice control (CON) or DHA-containing diet, subjected them to a single acute intranasal instillation of 2.5 mg cSiO2, then compared pulmonary inflammatory/autoimmune responses and autoimmune-related gene expression in experimental cohorts terminated at 7 and 28 d post-instillation (PI). Acute cSiO2 exposure of CON-fed mice elicited decreased macrophage and increased neutrophil numbers at 7 d PI, whereas at 28 d PI, CON-fed mice treated with particle displayed elevated total cell, macrophage, neutrophi..., NanoString autoimmune gene profiling
RNA was extracted from day 7 and day 28 lung tissue (n=4 samples/gp) using Tri Reagent (Sigma Aldrich, St. Louis, MO) and TissueLyser II (Qiagen). Extracted RNA was purified using a Zymo RNA Clean and Concentrator Kit with DNase according to the manufacturer's instructions (Zymo Research, Irvine, CA, cat no- R1017). The resulting RNA was dissolved in nuclease-free water and quantified using the Qubit (Thermo Fisher Scientific). RNA integrity was assessed using the Bioanalyzer (Agilent Technologies) at the MSU Genomics Core. Samples with RNA integrity >8 were analyzed using NanoString Autoimmune Gene Expression assay (Cat # XT-CSO-MAIPI1-12) at the MSU Genomics Core. Assays were performed and quantified on the nCounter MAX system, sample preparation station, and digital analyzer (NanoString Technologies) according to the manufacturer’s instructions. Data were processed using the nSolver 4.0 advance analysis as described previously (6) using NanoSt..., , # Data from: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice

Author/Principal Investigator Information
Name: Dr. Preeti Chauhan
ORCID: 0000-0001-8806-9552
Institution: Children's Hospital of Philadelphia
Address: 3401 Civic Center Blvd, Philadelphia, PA 19104
Email:

Author/Associate or Co-Investigator Information
Name: Dr. James Pestka
ORCID: 0000-0003-4689-2756
Institution: Michigan State University
Address: 567 Wilson Rd, Office 4176, East Lansing, MI 48824
Email:

Author/Alternate Contact Information
Name: Dr. Abby Benninghoff
ORCID: 0000-0002-7993-0117
Institution: Utah State University
Address: 4815 Old Main Hill, AGRS 427, Logan, UT 84322-4815
Email:

Author/Alternate Contact Information
Name: Dr. Olivia Favor
ORCID: 0000-0001-9164-7077
Institution: Michigan State University
Address: 567 Wilson Rd, Office 4183, East Lansing, MI 48824
Email:

Author/Alt...