Genome sequencing and assembly for 2DS of bread wheat cv. Chinese Spring. Triticum aestivum cultivar:Chinese Spring

We developed a new high-throughput gold standard genome assembly workflow using a large-scale BAC library with a refined two-step pooling strategy and the Lamp assembler algorithm. By applying this workflow with a MTP BAC library for 2DS of bread wheat, most BAC sequences were assembled to be gapless. We corrected large mis-assemblies in IWGSC RefSeq v1.0 in addition to filling the majority of gaps.