Single-cell RNA sequencing reveals recruitment of the M2-like CCL8high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

Tumor-associated macrophages (TAMs) are a crucial factor in reprogramming the tumor microenvironment following radiotherapy. The mechanisms underlying this process remain to be elucidated. We used single cell RNA sequencing (scRNA-seq) to investigate the effects of hypofractionated radiotherapy on macrophages dynamics in a subcutaneous Lewis lung carcinoma (LLC) murine model. The subcutaneous LLC tumors were ioslated 72 hours after hypofractionated radiotherapy (RT) or mock treatment (NT, 3 tumor samples in 1). Singel-cell suspensions were prepared and analyzed by using scRNA-seq.