File S1 - Elevated Expression of AKR1C3 Increases Resistance of Cancer Cells to Ionizing Radiation via Modulation of Oxidative Stress

Supporting information. Figure S1, Characterization of AKR1C3 expression and effect on cell proliferation. (A) Comparisons of cell proliferation, based on AlamarBlue assay, among KY170R-shRNA1, -shRNA2, -shRNA3 and –scramble cells together with their parental KY170R and KY170 cells to explore the effect of AKR1C3 knockdown on the growth of cells. Each experiment was carried out at least in triplicate and repeated twice with the error bar less than 20%. (B) Survival curves for comparison of the effect of AKR1C3 knockdown (sh1, sh2 and sh3 vs. scr.) and (C) overexpression (AKR1C3 vs. pcDNA3.1) on the sensitivity of KY170R, KY170 cells and TE13R cells to irradiation, which were determined by colony-formation assay. Each experiment was carried out at least in triplicate and repeated twice. Figure S2, Representative scale-up analyses of the effect of AKR1C3 on irradiated tumor stained by immunochemical and HE staining. Figure S3, Mechanistic exploration of AKR1C3 involvement in radioresistance. (A) Representative microscopic view and (B) quantitative comparisons of nuclear abnormalities in KY170R-scramble v. -shRNA1 cells post 4 Gy IR treatment. (C) Comparisons of the effect of elevated expression of AKR1C3 on proliferation of KY170R cells treated by doxorubicin. Figure S4, Analyses of AKR1C3 expression in 28 surgical samples of esophageal carcinomas. Among them 20 tumor samples are radioresistant and 8 are radiosensitive. Table S1, Characterizations of 28 esophageal cancer patients.
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