A gephyrin-like protein links cell division and polar elongation in Corynebacteriales

To discover missing players in corynebacterial cell division we used mass-spectrometry based interactomics using formaldehyde as crosslinker, starting with the FtsZ membrane anchor SepF as a bait for co-immunoprecipitation (co-IP) of divisome members. For SepF interactome we carried out the co-IPs using anti-Scarlet antibodies in Cglu strains expressing either SepF-mScarlet or mScarlet. Co-IPs using anti-SepF were also performed from Cglu or SepF-mScarlet strains. GLP interactomes were analysed in two strains: Cglu and Cglu_Dglpr strains.