Data from: In vivo imaging of metabolic heterogeneity across three endpoints relevant to aggressive breast cancer

File list:

1. Figure 1 Raw Data and Scripts
- Figure 1a) These values were collected via optical spectroscopy of scattering phantoms. These files are .mat files.
-Raw data to create Fig 1a are in folders "Individual Bodipy Phantoms" and "Three Probe Mixture".
-MATLAB script to re-create Fig 1a is titled'Simulating_Imaging_ScatteringPhantom_measured.m'
- Figure 1b) These values were collected via optical spectroscopy of scattering phantoms. These files are .mat files.
-Raw data to create Fig 1b are in folder "Three Probe Mixture" and file "inversionresult_NBDG_custNBDG_noHb_488nm.mat"
-MATLAB script to re-create Fig 1b is titled 'Simulating_Imaging_ScatteringPhantom_measured_NBDG.m'
- Figure 1c) These values were collected via optical imaging of scattering phantoms. These files are .tiff files.
-Raw data to create Fig 1c are in folders "20240313 background", "Bodipy Individual", "Vary Bodipy" and "Vary Bodipy Constant NBDG"
-MATLAB script to re-create Fig 1b is titled 'ScatteringPhantoms_VaryBodipyunmixing.m'
- Figure 1d) These values were collected via optical imaging of scattering phantoms. These files are .tiff files.
-Raw data to create Fig 1d are in folders "20240313 background", "NBDG Individual", "Vary NBDG" and "Vary NBDG Constant Bodipy"
-MATLAB script to re-create Fig 1b is titled 'ScatteringPhantoms_VaryBodipyunmixing.m'
- "References" contains images of individual fluorophore preparation scattering phantoms used as references for Fig 1c and 1d
2. Figure 2 Scripts
- Three MATLAB scripts (.m files), each to re-create Figure 2 plot for each fluorophore (Bodipy, 2-NBDG, and TMRE)
- Data for Figure 2 is found in the file "Figure 2, 3, 4, and SI Raw Data"
3. Figure 4 Script
- Two MATLAB scripts (.m files), each to re-create Figure 4
- "cluster_VWD_3p_oneImage_UpdatedColors.m" creates heterogeneity maps from images (one image at a time)
-Inputs are co-registered .tiff images, which are found in the file "Figure 2, 3, 4, and SI Raw Data"
- "StackedBarChartsHeterogeneity_ThreeProbe.m" creates stacked bar charts showing heterogeneity data
-Inputs are co-registered .mat files, which are created by "cluster_VWD_3p_oneImage_UpdatedColors.m" and can be found in the file "Figure 2, 3, 4, and SI Raw Data"
4. Figure SI3 Script
- Two MATLAB scripts (.m files), each to re-create Figure SI3
- "cluster_VWD_oneImage.m" creates heterogeneity maps from images (one image at a time)
-Inputs are co-registered .tiff images, which are found in the file "Figure 2, 3, 4, and SI Raw Data"
- "StackedBarChartsHeterogeneity.m" creates stacked bar charts showing heterogeneity data
-Inputs are co-registered .mat files, which are created by "cluster_VWD_oneImage.m" and can be found in the file "Figure 2, 3, 4, and SI Raw Data"
5. Figure 2, 3, 4, and SI Raw Data
- Raw data contained in two .zip files for Figures 2, 3, 4, and SI. These data were collected via wide field fluorescence optical imaging of Balb/c mice, either healthy mammary fat pads ("Normal") for orthotropic 4T1 tumors ("Tumor). These files are .tiff files.