A Method for Large-scale Identification of Protein Arginine Methylation

PASSEL ID: PASS00081. Original data file: B100910_042.mzXML. Data published as part of Mol Cell Proteomics. 2012 11:1489-99 . From the Abstract: {{i}} ... Here we show that most tryptic peptides containing methylated arginine(s) are highly basic and hydrophilic. Consequently, they could be considerably enriched from total cell extracts by simple protocols using either one of strong cation exchange chromatography, isoelectric focusing, or hydrophilic interaction liquid chromatography, the latter being by far the most effective of all. These methods, coupled with heavy methyl-stable isotope labeling by amino acids in cell culture and mass spectrometry, enabled in T cells the identification of 249 arginine methylation sites in 131 proteins, including 190 new sites and 93 proteins not previously known to be arginine methylated. ...{{/i}}