Additional file 2: of Interleukin-15 facilitates muscle regeneration through modulation of fibro/adipogenic progenitors

Figure S1. Flow cytometric gating strategy for isolation FAPs using FACS. Figure S2. The number of FAPs dropped quickly after 3 dpi, IL-15 can promote the growth of FAPs. (a) Representative immunofluorescence images of TA sections after glycerol injection. (b) Quantification of number of FAPs in TA muscles after administration of IL-15. (c) The growth of FAPs can be stimulated by IL-15 in vitro. Figure S3. IL-15 enhances collagen deposition in muscle with CTX injection. (a) A shcematic showing the experiment in vivo: IL-15 was administered from 1dpi to 3 dpi after CTX injection and samples were sectioned on 10 dpi. (b) Immunofluorescence for collagen I after IL-15 injection in injured muscle 5 dpi. Scale bar, 20Îźm. (c) Quantification of collagen deposition area (shown in percentage). (ZIP 2164 kb)