Coyotes take advantage of ungulate carrion subsidies as wolves recolonize Washington

Coyote (Canis latrans) scats were collected from two sites in northern Washington, USA. Scats were primarily collected from transects that were surveyed monthly, with additional scats collected opportunistically at both sites during winter (January to March) 2018, summer (mid-June to mid-September) 2018, and winter (January to March) 2019. Scats were stored frozen, sterilized at -80 degrees Celsius for two weeks, and then species of origin was determined by molecular analysis of mitochondrial DNA. Scats attributed to coyotes were washed until only undigested remains were left and allowed to air dry. To identify diet items, any hair, bones, teeth or claws were compared to a reference collection of skins, skulls, and hairs of all mammal species occurring in the study area, as well as to identification guides. In addition, medulla casts, hair length, color pattern, and diameter, which was measured at the mid-point of the hairs with the Nurugo Micro Smartphone Microscope, were also used to help identify species of origin. In addition to species identification, we also identified ungulate hairs from summer scats as either adult or juvenile. However, because of the similar color patterns and lengths, we could not reliably distinguish deer fawn hairs from elk calf hairs where their ranges overlapped. Ungulate age class was determined for summer-collected scats only. Once all items in the scat had been identified and sorted, we estimated the percent volume of each item to the nearest percent. This estimate was primarily based on the volume of hair present. Trace items under 1% of volume were omitted since they could represent contamination from the ground or the washing process. The total volume of all items summed to 100% for each scat (calculated excluding trace items).