Antibody repertoire of horse-derived V-segments

As part of generating equine antibody engineering tools, a novel primer set, EquPD v2020, based on equine V-gene data, was designed for efficient and accurate amplification of rearranged horse antibody V-segments. The primer set served for generation of immune phage display (PD) libraries, representing highly diverse V-gene repertoires of horses immunized against botulinum A or B neurotoxins. To demonstrate the comprehensive nature of the libraries, high-throughput sequencing (HTS) analysis was applied to map the Ab repertoires of the horses-derived V-segments represented in the libraries.