mRNASeq of z_Q175 knock-in mouse lines with and without neo cassette

In order to determine the effect of neoR gene in Q175 locus, mRNASeq was performed to compare the transcriptional signatures of zQ175 knock-in neo-in (z_Q175 KI) (CHDI-81003003) and zQ175 KI neo-out (Z_Q175 (neo -) KI) (CHDI-81003019) mouse lines. The z_Q175 KI is a knock-in mouse line with humanized exon 1 (190-200 pure CAG repeats) derived from Q140 KI colony. It contains floxed neo cassette upstream of exon 1. The Z_Q175 KI (neo -) KI was generated by crossing the Z-Q175 KI line with a zp3-cre transgenic line to delete out the neo cassette. mRNASeq was performed on samples from z_Q175 KI (CHDI-81003003) and Z_Q175 (neo -) KI (CHDI-81003019) mice and corresponding wild type littermate controls. Striatum, cortex, and liver tissues were collected N = 8 per group (4M/4F).