Gene expression data from 1 year and 16 weeks old SAMP8 and SAMR1 mice brain cortex.

Gene expression profiling reveals age dependent functional alternation in SAMP8 mice brain. We evaluated the effects of aging on SAMP8 mice brain. We performed an untargeted whole-genome transcriptome analysis to explore functionality age dependent changes of brain in SAMP8 mice. According to the manufacturer's guide, the RNA was extracted using Isogen kit (Nip-pon Gene Co. Ltd., Japan). Then, RNA quantity and quality were determined using the NanoDrop 2000 spectrophotometer (ThermoScientific, USA). RNA samples from cortices were amplified and labeled with the Affymetrix 3' IVT PLUS Reagent Kit, following the manufacturer's instructions. The labeled RNA was then hybridized onto Affymetrix Mouse Genome 430 PM array strips for 16 hours at 45°C using a hybridization station. After hybridization, the arrays were washed, stained, and scanned using the GeneAtlas Fluidics and Imaging Station. The probe intensity files (CEL files) were then processed using the robust multichip analysis (RMA) summarization algorithm in the Expression Console software (Affymetrix) to obtain gene-level information (CHP files). Further analysis of the data was conducted using Transcriptome Analysis Console (TAC) ver.4 software (Thermo Fisher Scientific). Finally, genes that passed the filter criteria of p value < 0.05 (one-way between-subjects ANOVA) and fold change > 1.1 (in linear space) were considered as differentially expressed genes (DEGs).