Time-Resolved Epifluorescence Wide-Field Imaging of Gastruloids and Trunk-Like Structures

Epifluorescence wide-field imaging of developing gastruloids and trunk-like-structures carrying a T::H2B-mCherry and Sox2::H2B-Venus reporter. Gastruloids and trunk-like-structures are mouse embryonic stem cell based embryo models (SEMs) of the embryonic trunk (Veenvliet et al., 2020). SEMs were cultured in separate wells and imaged over 4 days, every 24h.