Molecular profiling of DDC-induced biliary injury in mouse liver, RNA-seq

To assess changes in enhancer activity, chromatin accessibility and gene expression in hepatocytes versus cholangiocytes following 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) induced biliary damage in mouse liver.Mouse hepatocytes (Hep) as well as Epcam-positive biliary epithelial cells (Epcam) were isolated from mouse liver tissue of adult male animals (C57BL/6 strain, 18-22 weeks of age) kept on normal chow (Untreated) or subjected to an 11 day regimen of diet-based DDC treatment, followed by molecular profiling of gene expression (by RNA-seq), chromatin accessibility (by ATAC-seq) or histone H3 lysine 27 acetylation (H3K27ac, by ChIPmentation-seq)