Study of the mycobiota of the canine interdigital fold with pododermatitis by next generation sequencing

The yeast Malassezia pachydermatis is a common inhabitant of the skin and mucosae of dogs. However, under certain circumstances the yeast can overgrow and act as a secondary pathogen causing otitis and dermatitis in dogs. Canine pododermatitis is a common disease in dogs in which M. pachydermatis acts as a secondary pathogen. In the present study, the presence of Malassezia was assessed and quantified by different methods like cytological examination, culture, qPCR, and next generation sequencing (NGS). Samples from dogs with pododermatitis, healthy dogs and dogs after treatment were included.