Microarray analysis of mRNA expression in E14.5 and E16.5 mouse ovaries with and without Coup-tfII (Nr2f2). Mus musculus

COUP-TFII (NR2F2) is expressed in somatic cells in fetal ovary. To investigate the function of COUP-TFII , we used Cre-flox model to ablate Coup-tfII in the fetal ovaries We performed microarray to compare gene expression profile between control and knockout ovaries. Overall design: We established a tamoxifen inducible WT1-CreERT2; COUP-TFII flox/flox model to ablate Coup-tfII by injecting tamoxifen (2 mg/10g body weight) at E11.5 and E12.5. Control and knockout ovaries were collected at E14.5 and E16.5 for RNA extraction and hybridization on Affymetrix microarrays.