Seurat_AML.rds

This Seurat object contains single-cell RNA-sequencing data from <i>van Galen, P. et al., Cell, 2019</i> (https://doi.org/10.1016/j.cell.2019.01.031).<br>The dataset includes gene-expression profiles from 45 human samples, encompassing normal bone marrow, primary acute myeloid leukemia (AML) specimens, and cell lines (BM1, BM2, BM3, BM4, BM5.34p, BM5.34p38n, AML1012.D0, AML210A.D0, AML419A.D0, AML916.D0, AML921A.D0, AML314.D0, AML314.D31, AML371.D0, AML371.D34, AML475.D0, AML475.D29, AML722B.D0, AML722B.D49, AML870.D0, AML870.D14, AML997.D0, AML997.D35, AML329.D0, AML329.D20, AML329.D37, AML420B.D0, AML420B.D14, AML420B.D35, AML556.D0, AML556.D15, AML556.D31, AML328.D0, AML328.D29, AML328.D113, AML328.D171, AML707B.D0, AML707B.D18, AML707B.D41, AML707B.D97, AML707B.D113, MUTZ3.fresh1, MUTZ3.fresh2, MUTZ3.frozen, OCI.AML3).<br>The dataset comprises 44,823 cells and 27,899 genes. Metadata fields include malignant cell-type predictions and cell type annotations ("orig.ident", "NumberOfReads", "CyclingScore", "CyclingBinary", "MutTranscripts", "WtTranscripts", "PredictionRefined", "CellType", "nCount_RNA", "nFeature_RNA").<br><b>Organism:</b> <i>Homo sapiens</i><b>Assay type:</b> Seq-well single-cell RNA-sequencing.<b>Data type:</b> Processed single-cell gene expression and metadata (Seurat object, version 4.9.9083).<b>Related publication:</b> van Galen <i>et al.,</i> Cell 176, 1265-1281 (2019).<b>Raw data availability:</b> FASTQ and count matrices are available from GEO (accession GSE116256).