Results of the bacteriological culture and of the real time amplification assays for the tissue samples analyzed in the present study.

1Set Actin targets the β-actin gene of mammals,2Set 1 targets the lipL32 gene of pathogenic Leptospira;3Set 2 targets the secY gene of L. interrogans;4Set 3 targets the ompL1 gene of L. borgpetersenii;5Set 4 targets the secY gene of L. kirschneri;6Set 5 targets the secY gene of L. noguchii;7The analysis of the partial sequences of the secY gene of each isolate allowed to identify the Leptospira species; Amplification (+) or no amplification (−).Results of the bacteriological culture and of the real time amplification assays for the tissue samples analyzed in the present study.