Comparative gene expression profiles of NOS1-KO and WT A375 melanoma cell lines

NOS1 plays a vital role in tumor. A cell model of NOS1 gene knockout in human melanoma cell line A375 was constructed using CRISPR/Cas9 technique for the study of its function. We used microarrays to detail the Global gene expression underlying NOS1-knockout compare to wild type A375 cell. NOS1-KO and WT A375 cells for RNA extraction and hybridization on Affymetrix microarrays. There were three biological replicates performed for each cell lines.