Chromatin states and transcriptome of CD8+ T cells over the course of differentiation

The goal of this study is to analyze the contribution of transposable elements (TEs) tocis-regulation in CD8+ T cells. Using a combination of NGS techniques we show that specific subfamilies of TEs are enriched, and distributed in a lineage-specific fashion in core and boundary domains of CD8+T cell enhancers.This study represents the first detailed analysis of the topology and the enhancer domain-associated putative functions of distinct TE types. Overall design: ATAC-Seq and ChIP-Seq for H3K4me3 (in replicates), and total RNA-seq (in replicates or triplicates) performed in CD8+ T cells, naive (D0) and activated in vitro during one day (D1) and three days (D3).