Revealing the critical role ofin-situ plant and microbecommunity structure inremediation of typicalhigh-arsenic soil throughmolecular analysis

In situ plant-fungal combined remediation technology for arsenic (As)contaminated soil has emerged as a dominant technology in soil pollutiorremediation both domestically and internationally. However, the lack osystematic studies on in situ plants and rhizosphere fungal diversity in Ascontaminated soils, particularly in heavily polluted area, limits the application othe plant-fungal combined remediation technology. In this study, we surveyecand identified the distribution of dominant native plant in highly arseniccontaminated area, and then we used 18S rDNA technology to analyze thediversity of rhizosphere fungi and related factors from the area. The resultsrevealed that Pteris vittata (L.) of Pteridaceae and lmperata cylindrica (L.) Beauv. oPoaceae are the dominant native plants in highly arsenic-contaminated area. The concentrations of As in the rhizosphere soils of the dominant plants in the areaexceeded the As soil limits set by the European Union and the World HealthOrganization. A large quantity of As resulted in the dominance of fungi from the Glomeromycota, Ascomycota, and Basidiomycota in the contaminatecarea soils, while relative abundance of fungi is varied in different sitesAdditionally, soil acidity and alkalinity (pH), available phosphorus (AP), and Ashad the most notable effects on fungal diversity in Shihuangsi village ancLinkuang village, whereas the low soil organic carbon (SOC) content in Hesharvillage was the primary limiting environmental factor for fungal diversity. Theresults of this study provide a theoretical foundation and technical guidance fothe development of novel plant-fungal combined remediation technologie.aimed to the control of As pollution in plant and soil.