Comprehensive Analysis of the Translatome in High Fat Diet-Induced Liver Steatosis

We performed translatome and transcriptome sequencing (RNC-seq and RNA-seq) on the livers of normal chow diet-, high-fat diet- and High-fat diet plus lycopene-fed mice. Overall design: Total RNA and ribosome-bound RNA were extracted from the livers of normal chow diet- (control), high-fat diet- (HFD) and High-fat diet plus lycopene (HFD+LYC)-fed mice (three biological replicates per condition), and the polyA+ mRNA was sequenced using Illumina HiSeq X Ten sequencer. The reads were mapped to RefSeq RNA reference sequences and the expression level were quantified by rpkM calculation.