超快上转换荧光显微镜成像技术指标数据集

时间分辨荧光检测是用于判断物质激发态寿命和载流子动力学过程的常用技术之一。同时具备时间-空间分辨的荧光动力学成像显微镜主要用于研究半导体微纳尺度样品，光电转换材料，光催化材料以及生物样品等微纳空间尺度内的荧光动力学过程及成像。然而，传统的时间分辨荧光成像设备通常基于TCSPC技术实现，其时间分辨率较低，难以满足超快动力学过程的探测需求。因此，我们利用超快上转换荧光光谱探测技术结合微区扫描成像，设计构建超快上转换荧光显微镜成像系统，从而实现超快荧光的显微成像测量。
在该系统中，激光器产生的飞秒脉冲被分为两束，其中一束作为泵浦光激发样品产生荧光，另一束作为门脉冲与荧光通过非线性光学晶体产生和频，和频光经过分光后进入检测器记录强度信息；其中，泵浦和门脉冲间的时间延迟通过光学延迟线控制。
本数据集主要面向超快荧光动力学成像研究领域，利用飞秒上转换荧光技术设计具备超高时空分辨能力的超快上转换荧光显微镜成像系统，记录了在性能指标测试中获得的原始数据和分析数据，数据体量为1.22 MB。

Time-resolved fluorescence detection is one of the most commonly used techniques for determining the excited-state lifetime and carrier dynamics of materials. Time- and space-resolved fluorescence dynamic imaging microscopes are primarily employed to investigate fluorescence dynamic processes and imaging at micro/nanoscale scales, including semiconductor micro/nanoscale samples, photoelectric conversion materials, photocatalytic materials, and biological samples. However, traditional time-resolved fluorescence imaging equipment is typically implemented based on TCSPC technology, which exhibits relatively low temporal resolution and fails to meet the detection requirements of ultrafast dynamic processes. Therefore, we combined ultrafast upconversion fluorescence spectroscopy detection technology with micro-area scanning imaging to design and construct an ultrafast upconversion fluorescence microscope imaging system, enabling microscopic imaging measurement of ultrafast fluorescence.
In this system, the femtosecond pulses generated by the laser are split into two beams. One beam serves as the pump light to excite the sample and induce fluorescence emission, while the other beam acts as the gating pulse to generate sum-frequency generation with the fluorescence through a nonlinear optical crystal. The generated sum-frequency light is spectrally split and then directed into a detector to record the intensity information. The time delay between the pump and gating pulses is controlled by an optical delay line.
This dataset is primarily targeted at the research field of ultrafast fluorescence dynamic imaging. It is derived from an ultrafast upconversion fluorescence microscope imaging system designed with femtosecond upconversion fluorescence technology, which features ultra-high spatiotemporal resolution. The dataset records both raw data and analyzed data obtained during performance index tests, with a total data volume of 1.22 MB.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于超快荧光动力学成像领域，通过飞秒上转换荧光技术构建了一个具备超高时空分辨能力的显微镜系统，用于记录性能指标测试的原始和分析数据。数据集体量较小（1.28MB），包含9个文件，格式包括PDF、CSV等，适用于微纳尺度样品的光电材料研究。