Pushing the Boundaries of Resolution in Solid-State Nuclear Magnetic Resonance of Biomolecules with 160 kHz Magic-Angle Spinning

We present ultrafast
magic-angle spinning (MAS) at 160 kHz in solid-state
nuclear magnetic resonance (NMR), demonstrating unprecedented spectral
quality and coherence lifetimes in proton-detected experiments on
biomolecular systems. By optimizing experimental and sample conditions
and utilizing advanced filling and handling tools, we achieve superior
resolution on both microcrystalline and membrane-reconstituted proteins,
paving the way for new applications in structural biology.