Genome-wide profiling for H2A.Z and genome accessibility using ATACseq in Uhrf1hepKO mouse livers compared to WT livers

This study was carried out to investigate potential changes in the genome-wide distribution of H2AZ and genome accessibility using ATACseq in livers where Uhrf1 is deleted in hepatocytes (Uhrf1-fl/fl;Alb:Cre) compared to controls (Uhrf1-fl/fl). Overall design: ChIP was carried out to pull down H2AZ bound chromatin from nuclei isolated from s single Uhrf1-Fl/fl liver and a single (Uhrf1-fl/fl;Alb:Cre) male liver between 8-12 weeks of age. This chromatin fraction was then digested using micrococcal nuclease to release mononucleosomes. DNA fragments purified from nucleosomes were then used to generate libraries and sequenced. The same livers were used for chromatin accessibility using the ATAC-seq Protocol as described in Buenrostro et al (2015).