stRNA-seq of brain glioblastoma sample

Proneural-mesenchymal transition (PMT) is a phenotypic alteration and contributes to therapeutic resistance and recurrence of glioblastoma (GBM). Macrophages, as a main infiltrating component of tumor immune microenvironment (TIM), can regulate the biological processes of PMT. However, the mechanisms driving this process remain largely unknown. Here, We performed single-cell RNA sequencing (scRNA-seq) (3V3) and Spatial transcriptomics RNA sequencing(stRNA-seq)(1V1) from tumor core and matching tumor periphery samples to discripe the overall landscape of tumor and nontumor cells in gliomas. We profiled freshly isolated cells from tumor core and paired surrounding peripheral tissue in glioma patients. scRNA-seq (3V3) and stRNA-seq(1v1) were used to discripe the overall landscape of tumor and nontumor cells in gliomas.