Histone deacetylation primes chromatin to preserve epigenetic memory for self-propagation of heterochromatin domains [H3-T7 ChIP]

ChIP-chip analyses of pulse-expressed H3-T7 in clr3-CDx2 and wild type cells Chromodomain-mediated HDAC loading suppresses histone turnover at self-templated heterochromatin domain. Chromodomain-mediated loading targets Clr3-CDx2 to heterochromatin domains. DNA co-purified with H3-T7 immunoprecipitated chromatin was random-prime PCR amplified and analyzed using custom 60mer Agilent array that tiles Schizosaccharomyces pombe genome in 300bp intervals.