Additional file 3 of A genome-wide study of PDZ-domain interactions in C. elegans reveals a high frequency of non-canonical binding

Additional file 3:PDZome network. Additional file 3 contains four tables (S3 to S6) listing the PDZome network interacting pairs and giving statistical analysis of the interactions. Supplemental Table S3: Two hybrid screen results. Gene names and ID are given according to Wormbase WS150[44]. Number of hits refers to the number of independent colonies identified and phenotypically tested for each interacting partner. Number of splice-forms identified or predicted in Wormbase WS150 and last 6 amino acids of each splice-form are given in cases were several splice-forms are identified or predicted. When sequencing from the N-terminus did not span the entire fragment, and thus the C-terminus was not experimentally confirmed, if any of the predicted splice-form had a C-terminal consensus motif, to be conservative, a consensus class was attributed. Consensus class type: [ST]X[YFWCMVILA] = 1; [YFWCMVILA]X[YFWCMVILA] = 2; [DE]X[YFWCMVILA] = 3 (X: any amino acid). Supplemental Table S4: Promiscuity and specificity of PDZ interactome network. Number of independent interacting proteins per PDZ domain, and number of PDZ domains interacting with each protein are given for AD-wrmcDNA and AD-ORFeome libraries two-hybrid screens. Supplemental Table S5: Promiscuity and specificity of PDZ interactome network: mean and median for number of interacting proteins per PDZ or vice versa. Supplemental Table S6: Number of interacting proteins per consensus class in network. Single hits: interacting proteins for which only one clone was identified in two-hybrid screens. Multiple hits: interacting proteins for which more than one clone was identified in two-hybrid screens. (XLS 206 KB)