HLA matching or CRISPR editing of HLA Class I/II enables engraftment and effective function of allogeneic human regulatory T cell therapy in a humanized mouse transplantation model

Immunodeficient BALB/c Rag2-/- cγc-/- mice grafted with human skin received intraperitoneal human PBMCs (5x106) either alone (n=4) or with autologous (n=4), allogeneic (n=4), or H/C edited (n=4) Tregs (1 x 106). Grafts were monitored for macroscopic signs of rejection and were harvested at 15 days following adoptive transfer.Spatial mapping of 427 transcripts was performed across five skin grafts (one each from experimental group treated with PBMC alone, autologous Treg, or allogeneic Treg, and two from the experimental group treated with H/C Treg). The raw data bundle from the Xenium Analyser is provided.