ddRAD-Seq of Cowpea (Vigna unguiculata (L.) Walp) unguiculata

This study uses double digest restriction-site associated DNA sequencing (ddRAD-Seq) to construct a genetic fingerprinting map for cowpea (Vigna unguiculata), a key leguminous crop. Genomic DNA from diverse cowpea accessions was sequenced to identify stable, polymorphic molecular markers, enabling the establishment of a standardized fingerprinting system.This work provides a reliable tool for cowpea germplasm identification, avoids genetic resource confusion, and lays a foundation for subsequent marker-assisted breeding and genetic diversity research, supporting efficient conservation and utilization of cowpea genetic resources.