Parathyroid Hormone Treatment Effect on Gene Expression in Primary Calvarial Osteoblasts

The effect of parathyroid hormone (PTH) treatment was tested on osteoblasts prepared from calvaria of C57BL/6J mice 7-10 days old. Osteoblasts were treated with either human parathyroid hormone (1-34), bovine (D-Trp12,Tyr34) parathyroid hormone (7-34) amide or vehicle control for 4 hr. Gene expression effects were evaluated by DNA microarray. Mouse osteoblasts were prepared independently for each biological replicate. Three biological replicates were used for each treatment. Osteoblasts were treated with either 100 nM human parathyroid hormone (1-34), 1 µM bovine (D-Trp12,Tyr34) parathyroid hormone (7-34) amide or vehicle for 4 hr. Total RNAs were collected and 100 ng analyzed for gene expression using Affymetrix Mouse Gene 2.0 ST GeneChips.