Comprehensive Transcriptome Analysis of lncRNAs Reveals the Role of lncAD in Chicken Intramuscular and Abdominal Adipogenesis

Adipose tissue-specific distribution
and deposition speed are the
main factors affecting the slaughter performance and meat quality
in poultry. Previous studies suggested that different adipose tissues
owned various biochemical characteristics and gene expression patterns.
To investigate the functional role of long noncoding RNAs (lncRNAs)
during chicken intramuscular and abdominal adipogenesis, we performed
transcriptome analysis by Ribo-Zero RNA-Seq technology. A total of
11247 lncRNAs were observed in the adipocytes derived from IMF and
AbF in chicken. Among them, we got 1624 differentiated expressed novel
lncRNAs. A large amount of lncRNAs were involved in several lipid
metabolism and adipogenesis-related signaling pathways. Of these,
lncRNAs, lncAD is one of the most upregulated lncRNA and was coexpressed
with several genes of the PPAR signaling pathway. Here, we report
that knockdown of lncAD inhibited its upstream gene TXNRD1 expression in a cis-regulation manner, thus to
decrease intramuscular preadipocytes adipogenic differentiation and
promoted cell proliferation. Our present study revealed huge lncRNAs
profile differences between IMF- and AbF-derived preadipocyte adipogenesis.
Collectively, our findings not only provide valuable evidence for
the identification of adipogenic lncRNAs but also contribute to further
studies about the post-transcriptional regulation mechanism underlying
tissue-specific fat deposition in poultry.