Transcriptional profiling of CTLA-4 as well as TGFb treated CD4+ T cells vs. a Treg cell phenotype and Tconv cells

Comparison of the transcriptional profile of CTLA-4 as well as TGFb treated CD4+ T cells with a Treg cell phenotype and Tconv cells. Since CTLA-4 and TGFb have been described as regulatory T cell inducing factors, CD4+ T cells were activated in the presence of CTLA-4 as well as TGFb. The transcriptional profile of these cells was compared to untreated CD4+CD25- T cells as well as to regulatory T cells in order to determine the relationship of these treated cells to regulatory T cells. Freshly isolated CD4+ T cells, CD4+CD25- as well as CD4+CD25+ Treg cells were treated as indicated and afterwards analysed by perfoming genome-wide expression analysis.