RNA-seq Analysis of mRNA profiles in Purß-overexpressing and Purß-knocking down hepatocytes

Purpose: The goal of this study is to investigate how Purß regulates hepatic glucose production in primary hepatocytes. Methods: mRNA profiles of Purß-overexpressing, and Purß-knocking down hepatocytes were generated by deep sequencing using an Illumina NovaSeq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(Ensemble_GRCm38.96) with TopHat (version 2.0.12), and the aligned reads were used to quantify mRNA expression by using HTSeq-count (version 0.6.1). Conclusion: Our study represents the first detailed analysis of mRNA profiles in Purß-overexpressing, and Purß-knocking down hepatocytes, generated by RNA-seq technology. Our results identified Adcy6 was a target of Purß. RNA-seq analysis showed that Purß overexpression increased Adcy6 expression, whereas Purß knockdown decreased Adcy6 expression. Overall design: mRNA profiles of Purß-overexpressing, and Purß-knocking down hepatocytes were generated by deep sequencing using an Illumina NovaSeq 6000 platform.