Raw data from sodium current recordings obtained from tagged human sodium channels (Nav1.5) expressed in HEK293 cells.
收藏f1000.figshare.com2023-06-01 更新2025-03-21 收录
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For each channel type the cells are numbered starting from 1. For the sake of similar conditions (cells from the same passage, recordings on the same day, same solution aliquots), new cells have been recorded for the tag study. There are two sheets per channel type: one with data from the current voltage (I/V) activation, together with the steady-state inactivation (SSI) protocol; and a second one with data obtained with the onset of slow inactivation (OSI) and the recovery from inactivation (RFI) protocol. For some cells, only some of the protocols have been applied (thus non-continuous cell numbering in some OSI/RFI sheets). All measured voltages are given in mV; cell capacitance was measured in pF. Note that the cells transfected with untagged hNav1.5 used for comparison with mNav1.5 are not the same as in the study with different tags. See Figure 2 and 4 for information about the applied protocols.
针对每一种信道类型,细胞编号均从1开始。为确保实验条件的一致性(来自同一片段的细胞、同一天进行的记录、相同的溶液分装),针对标记研究,对新的细胞进行了记录。每个信道类型包含两张表格:一张包含当前电压(I/V)激活和稳态失活(SSI)协议的数据;另一张则包含缓慢失活(OSI)和失活恢复(RFI)协议获得的数据。对于某些细胞,仅应用了部分协议(因此某些OSI/RFI表格中细胞编号不连续)。所有测量的电压单位均为毫伏(mV);细胞电容的测量单位为皮法拉(pF)。请注意,与mNav1.5进行对比的未标记hNav1.5转染的细胞与使用不同标记的研究中的细胞不同。具体协议的应用信息,请参考图2和图4。
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