Chromatin isolation by RNA purification sequencing of LINC02015 in human aortic smooth muscle cells

This study was designed to explore the role of LINC02015 in regulating biological behaviors of vascular smooth muscle cells (VSMCs) and investigate the interacting targets of LINC02015 with high-throughput techniques and bioinformatics analysis. Chromatin isolation by RNA purification (ChIRP) sequencing was applied to explore the interaction relationship among LncRNA, RNA-binding protein and DNA. Localization of the active binding sites was specified by high-throughput sequencing with STAR2. Peak calling analysis was applied to spot enriched gene peaks with MACS. Overall design: Examination of LINC02015 binding sites in human aortic smooth muscle cells