Next Generation Sequencing Analysis of human hematopoietic stem/progenitor cells (HSPCs) differentiated from pluripotent stem cells

Purpose: global gene expression profiling of H1 and iPSC derived HSPCs. Methods: SMART-seq2 amplified polyA RNA from differentiation day 8 CD31+CD34+CD43+ and CD31+CD34+CD43- cells, and day 5 CD31+CD34+ cells cultured in medium with or without insulin supplement Results: Modulation of key cell signaling pathways lead to highly efficient HSPC differentiation from human pluripotent stem cells. Conclusions: Highly efficient HSPC differentiation from human pluripotent stem cells in a monolayer, chemically defined culture system. Overall design: Perform RNA-seq of polyA RNA from differentiation day 5 CD31+CD34+ and day 8 CD31+CD34+CD43+ and CD31+CD34+CD43- cells derived from H1 and iPSCs