Crowder-specific modulation of hepatitis C virus NS3/4A protease activity and local structural dynamics

Data: RP-HPLC chromatogram of synthesized FRET-substrate; EDANS fluorescence calibration in dilute conditions (no crowder) and in the presence of PEG 2000, PEG 4000, Ficoll 70, Dextran 70, and lysozyme; FRET-substrate fluorescence in the absence and presence of 500 nM EDANS; fluorescence kinetics of FRET-substrate spontaneous cleavage in dilute conditions and HEWL-mediated cleavage in the presence of lysozyme; fluorescence kinetics of FRET-substrate cleavage by NS3/4A measured in dilute conditions and in the presence of PEG 2000, PEG 4000, Ficoll 70, Dextran 70, and lysozyme; NS3/4A intrinsic fluorescence measured across 30-65°C in dilute conditions and in the presence of PEG 2000, PEG 4000, Ficoll 70, and Dextran 70.