GNPS - Human NVU - Endothelial cells - Methamphetamine
收藏NIAID Data Ecosystem2026-03-11 收录
下载链接：
https://www.omicsdi.org/dataset/gnps/MSV000085789
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资源简介：
For human brain microvascular endothelial cells, we used adult passage 3 cortical cells (hBMVECs,
CellSystems, Kirkland, WA, USA). Cells were seeded at 13-15000 cells/cm2 in CSC complete
medium (Cell Systems, Kirkland, WA, USA) on tissue culture plastic culture dishes coated
with Attachment factor for 30 min (Cell Systems, Kirkland, WA, USA).
Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was
prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were
made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs.
Further info in
"Proteomic and metabolomic characterization of human neurovascular unit
cells in response to methamphetamine" Herland et al Adv Biosystems 2020
创建时间：
2020-07-20
