Comparison of transcriptomes between Marf1+/+ (WT) and Marf1 ENU/ENU (Mutant) fully-grown oocytes (FGO)

Marf1 (MUT) female mice are infertile and the meiosis of the oocytes are arrest at prophase I. We thought to identify the potential causes of the meiotic arrest phenotype in the mutant oocytes by comparing the transcriptomes of the WT and mutant fully-grown oocytes (from 23-d old mice) that are transcriptional silent. We compared the transcriptomes of WT and MUT FGOs. 3 individual oocyte samples of each genotype (WT and MUT).Marf1（MUT）雌性小鼠表现为不育表型，其卵母细胞的减数分裂停滞于减数分裂I期前期。为解析突变型卵母细胞出现减数分裂停滞表型的潜在成因，本研究选取取自23日龄小鼠、处于转录静默状态的完全成熟卵母细胞（Fully-grown Oocytes，FGOs），通过对比野生型（Wild Type，WT）与突变型卵母细胞的转录组开展分析。本研究比较了两种基因型的完全成熟卵母细胞转录组，每种基因型（野生型与MUT）均设置3个独立的卵母细胞样本。