Identification of candidate genes simultaneously shared by adipogenesis and osteoblastogenesis from human mesenchymal stem cells

we aimed to screen candidate genes of adipocyte/osteoblastogenesis using human mesenchymal stem cells (hMSCs) at 3, 5, and 7 days through RNA-Seq technique. The 275 significantly differentially expressed genes (DEGs) were identified simultaneously shared by two anecdotes. Furthermore, we confirmed that ANKRD1 (ankyrin repeat domain 1) facilitated adipogenesis and weaked osteoblastogenesis via RNA interference (RNAi), and IGF1 (insulin like growth factor 1) simultaneously enhancted adipo-osteoblastogenesis. This study would provide the potential molecular switches for the study of the balance of adipo-osteoblastogenesis and therapy of metabolic bone disease osteoporosis. Overall design: Examination of 4 different sampling time in human mesenchymal stem cells.