Nitrogen enrichment buffers phosphorus limitation by mobilizing mineral-bound soil phosphorus in grasslands

Phosphorus (P) limitation is expected to increase due to nitrogen (N)-induced terrestrial eutrophication, although most soils contain large P pools immobilized in minerals (Pi) and organic matter (Po). Here we assessed whether transformations of these P pools can increase plant available pools alleviating P limitation under enhanced N availability. The mechanisms underlying these possible transformations were explored by combining results from a 10-year field N-addition experiment and a 3700-km transect covering wide ranges in soil pH, soil N, aridity, leaching, and weathering that can affect soil P status in grasslands. Nitrogen addition promoted dissolution of immobile Pi (mainly Ca-bound recalcitrant P) to more available forms of Pi (including Al- and Fe-bound P fractions and Olsen P) by decreasing soil pH from 7.6 to 4.7, but did not affect Po. Soil total P declined by 10% from 385±6.8 to 346±9.5 mg kg-1, while available-P increased by 546% from 3.5±0.3 to 22.6±2.4 mg kg-1 after 10-...