BCCZO -- Soil Microbes -- Gordon Gulch -- (2011-2011)

In this study conducted by Kathryn Eilers, distributions of Bacteria and Archaea were conducted within Upper and Lower Gordon Gulch. The purpose of this study is to discover how the distributions are related to slope aspect, vegetation type, and edaphic factors.

Sampling was conducted along five transects approximately 200m (transects 1, 2, 3, 6 and 7), 400m (transects 8, 9, and 10), or 600m (transects 4 and 5) in length in the two regions of the watershed (see Overview Map) with samples collected from twenty, regularly spaced points (10 m, 20 m, or 30 m apart) along each transect. Each sample consisted of twenty, 0-5 cm cores, taken within a one meter radius, that were homogenized and sieved to 2 mm. Samples were then stored at 40C and split for analysis within two days of collection. Samples used for soil microbial community analysis were frozen at -800C until DNA was extracted.