Additional file 2 of Functional metagenomics reveals differential chitin degradation and utilization features across free-living and host-associated marine microbiomes
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Additional file 1: Table S1. Protein family (Pfam) entries involved in chitin and chito-oligosaccharides degradation and N-acetylglucosamine utilization used in Figure 1. Table S2. 16S rRNA gene-based estimates of relative abundance of culturable bacterial genera in the microbiomes of octocorals and sponges. Table S3. Genome-based estimates of relative abundance for culturable, chitinolytic symbionts of sponges and corals in their respective microbiomes. Table S4. Features of 90 (nearly) full-length coding sequences displaying endo-chitinase catalytic domains (from glycoside hydrolase families 18 and 19, Pfam-based annotations) used for phylogenetic inferences in Figure 2. Table S5. InterPro (IPR) abundance of coding sequences involved in chitin and chito-oligosaccharides degradation and N-acetyl-glucosamine utilization in (a) the marine sponge metagenome dataset (project accession number: PRJEB11585) and (b) the octocoral metagenome dataset (project accession number: PRJEB13222), retrieved using the MGNify (EMBL-EBI) pipeline. Table S6. Endo-chitinase (EC 3.2.1.14) nucleotide sequences retrieved from (a) the marine sponge (Spongia officinalis) metagenome dataset (project accession number: PRJEB11585) and (b) the octocoral metagenome dataset (project accession number: PRJEB13222), using the MG-RAST metagenomics analysis server (version 4.0.3). Table S7. Genus-level taxonomy of endo-chitinase gene reads in the microbial metagenomes of sponges and corals.
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figshare
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2021-02-15



